Cytotoxicity of some preservatives for culture Chlorella Vulgaris GKO strain

¹ Federal State Budgetary Educational Institution of Higher Education “St. Petersburg State University of Veterinary Medicine”, St. Petersburg, Russia
² Federal State Budgetary Institution “National Medical Research Center named after V. A. Almazov”, St. Petersburg, Russia

^* Corresponding author: ilya.makh@mail.ru

Abstract

One of the ways to preserve the culture of microalgae for a long time is conservation. All modern methods of preservation and long-term storage of microorganism cultures are based on the transfer of cells to a state of anabiosis, in which metabolic processes are either completely or partially stopped. One of the mechanisms that are realized during the transition to the anabiotic state is a change in cell permeability. This can be achieved by modifying the culture medium. Thus, the search for preservatives that contribute to the long-term preservation of biomass is very relevant. The primary condition for the use of any preservative or a new component of the culture medium should be the absence of a toxic effect on the cell culture. Goal of the work: to determine the cytotoxic effect of some preservatives for the cell culture of Chlorella vulgaris GKO strain. The cytotoxicity of ascorbic acid, lactic acid, citric acid, acetic acid, sodium chloride and urotropin was analyzed for the cell culture of Chlorella vulgaris strain GKO. The following parameters were determined: pH of Chlorella vulgaris suspension with preservative; Total number of cells, MM/ml; The ratio of dead cells to the total number of cells,%; Specific growth/death rate; The optical density of the suspension; The difference in the average optical density,%; Cell size (diameter), µm pH of suspension of Chlorella vulgaris with preservative; Total number of cells, MM/ml; The ratio of dead cells to the total number of cells,%; Specific growth/death rate; The optical density of the suspension; The difference in the average optical density,%; Cell size (diameter), µm pH of suspension of Chlorella vulgaris with preservative; Total number of cells, MM/ml; The ratio of dead cells to the total number of cells,%; Specific growth/death rate; The optical density of the suspension; The difference in the average optical density,%; Cell size (diameter), µm