| Issue |
BIO Web Conf.
Volume 66, 2023
International Scientific and Practical Conference “AGRARIAN SCIENCE - 2023” (AgriScience2023)
|
|
|---|---|---|
| Article Number | 10004 | |
| Number of page(s) | 8 | |
| Section | Breeding, Selection and Genetics of Farm Animals | |
| DOI | https://doi.org/10.1051/bioconf/20236610004 | |
| Published online | 08 September 2023 | |
Improvement of the technique for the bovine genome editing using the example of knockout of the CD 209 receptor responsible to the bovine leukemia virus
Federal State Budgetary Scientific Institution Ural Federal Agrarian Scientific Research Centre, Ural Branch of the Russian Academy of Sciences (FSBSI UrFASRC, UrB of RAS), 112 А, Belinskogo St., 620000 Еkaterinburg, Russia
* Corresponding author: isaeva.05@bk.ru
The aim of this work was to evaluate the possibility of editing the CD209 gene associated with bovine leukemia virus (BLV) infection. Two ways of delivering the gene editing system into the zygote were investigated: delivery of the CRISPR/Cas9 system using a viral vector and injection of mRNA and sgRNA into the oocyte cytoplasm. The most effective was the microinjection of spCas9 protein mRNA and sgRNA targeting CD209 at the zygote stage into the cytoplasm.
© The Authors, published by EDP Sciences, 2023
This is an Open Access article distributed under the terms of the Creative Commons Attribution License 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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