Features of the development of strawberry microplants in vitro

St. Petersburg State Agrarian University, 2, Petersburg Highway, Pushkin, St. Petersburg, 196601, Russia

Abstract

This article presents the results of a study of the effect of different sterilizers and nutrient media of different composition on the characteristics of the growth and development of garden strawberry (Fragaria x ananassa) microplants in vitro. To introduce a plant into a cell and tissue culture, two very important conditions must be met - to choose the right sterilizing solution, its concentration and processing time, and to choose the right balanced nutrient medium suitable for a particular culture. The studies were carried out on two varieties of garden strawberries: Favorit and White Swede. Sterilization of plant material was carried out with two sterilizers - 5% sodium hypochlorite solution and 1% silver nitrate solution. Studies have shown that the largest number of sterile explants was obtained by sterilizing the material with silver nitrate. Subsequently, viable meristems were planted on three nutrient media of different composition. According to the data obtained, it can be concluded that the MS nutrient medium with the addition of cytokinin 6-BAP at a concentration of 1 mg/L turned out to be the best in terms of the multiplication factor, the percentage of survival and the height of microplants..