Effect of iron nanoparticles on germination and biometric parameters of fiber flax seedlings

Soil Institute. V. V. Dokuchaeva, 7, building 2b, Pyzhevsky per., Moscow, 119017, Russian Federation

^* Corresponding author: n.nemygina@gmail.com

Abstract

The article investigates the effect of iron nanoparticles introduced into the composition of a liquid-phase biological agent (LPB) on germination and biometric parameters, such as the average length and average weight of one seedling, fiber flax. Iron nanoparticles were obtained by green synthesis using a 0.5 mol/L FeCl3∙6H2O solution as a precursor and green tea extract as a reducing agent. The finished nanoparticles were introduced into LPB in a volume ratio of 1:50 to obtain the LPB-Fe biological agent. Both biological agents were tested during the germination of fiber flax seeds. Two ways of using biological agents were studied - seed watering and inoculation, while in both cases the concentration of biological agents varied from 0.05 to 1.0%. Water was used as a control. As a result, it was found that in the case of flax seeds, watering is more effective than inoculation. Thus, when irrigating seeds, the maximum germination rate of 99% was obtained using 0.4% LPB-Fe, and the maximum average length of one seedling was 14.4 ± 0.8 cm at a LPB-Fe concentration of 0.1%. Whereas, when seeds were inoculated in LPB-Fe, the maximum germination rate of 95% and seedling length of 13.9 ± 0.6 were obtained at a concentration of 0.2%. When irrigating with LPB, the highest germination rate of 96% was obtained at a biological agent concentration of 1.0%, and the maximum average length of one seedling of 14.5 ± 0.7 cm was obtained at a biological agent concentration of 0.6%. At the same time, none of the biological agents affected the average weight of one seedling.