Growing cell cultures of some rare local species of medicinal plants

¹ Osh State University, Osh, Kyrgyzstan
² Institute of Biomedical Problems, South Branch of the National Academy of Sciences of the Kyrgyz Republic, Osh, Kyrgyzstan

Abstract

The article analyzes the results of growing new planting materials by tissue culture of some medicinal plants (Eminium regelii Vved., Angelica ternate Regel et Schmalh., Aconitum rotundifolium Kar. et Kir., Gentiana algida Pall., Rodiola heterodonta (Hook. f. et Thomson) Boriss.), found on the territory of the natural park Kara-Shoro of Kyrgyzstan. The conditions for growing callus cultures by the in vitro method were studied and the temperature optimum for growing cell cultures in the laboratory was identified. Callus cultures were grown on four types of nutrient media, which differ from each other in their component composition, and the preparation technique is the same.In the course of the study, it was found that the most universal nutrient medium is the Murashige-Skoog medium, in which callus formation is relatively more observed. The nutrient medium of Nitsch and Nitsch showed the lowest indicators of crop survival. Gamborg’s and Eveleg’s media showed good results, but root formation proceeded comparatively faster in White’s nutrient medium. Planting schemes for medicinal plants and conditions for growing and development close to the ecological optimum have been developed. Based on the studies carried out, methods were proposed for creating a research and production complex for growing cell cultures of the State Natural Park (SNP) Kara-Shoro.