Issue |
BIO Web Conf.
Volume 80, 2023
4th International Conference on Smart and Innovative Agriculture (ICoSIA 2023)
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Article Number | 05001 | |
Number of page(s) | 8 | |
Section | Smart Genetics Resource Management and Utilization | |
DOI | https://doi.org/10.1051/bioconf/20238005001 | |
Published online | 14 December 2023 |
Continental vs. tropical breed: Immunity comparison under heat stress conditions utilizing qRT-PCR technique
1 Centre for Agricultural Genomics and Biotechnology, University of Debrecen, 4032 Debrecen, Egyetem tér 1, Hungary.
2 Department of Agriculture-Bureau of Animal Industry, 1100 Diliman, Quezon City, Philippines.
3 College of Veterinary Medicine, University of Southern Mindanao, Kabacan, Cotabato 9407 Philippines
4 Doctoral School of Animal Science, University of Debrecen, 4032 Debrecen Böszörményi út 138, Hungary.
5 Department of Animal Breeding and Reproduction, Faculty of Animal Science, Universitas Gadjah Mada, Yogyakarta 55281, Indonesia.
6 Department of Animal Biotechnology, Hungarian University of Agriculture and Life Sciences, Gödöllő, 2100, Hungary.
7 Department of Physiology, Veterinary Faculty, University of Sarajevo, Sarajevo, 71000, Bosnia and Herzegovina.
* Corresponding author e-mail address: kusza@agr.unideb.hu
Understanding the responses of animals to seasonal heat stress on the genomic level has led to the identification of genes implicated in thermal stress reaction mechanisms. In this study, the relative gene expression of Interleukin-10 (IL-10), an anti-inflammatory cytokine and biomarker for heat stress-mediated immune modulation, was observed during the summer and winter seasons in continental and tropical sheep breeds, namely, Hungarian Indigenous Tsigai and White Dorper, employing quantitative real-time polymerase chain reaction (qRT-PCR). Temperature-humidity indices (THI) were calculated to assess heat stress levels. The results indicate that IL-10 CT-values were significantly higher during the summer, when heat stress prevails, in both sheep breeds compared to winter. While the White Dorper exhibited a higher numerical value for the summer relative gene expression ratio (16.2) compared to the Hungarian Indigenous Tsigai (12.3), no significant differences in CT values were observed between breeds or among sexes. These findings suggest the immune-adaptive characteristics of the two sheep breeds during seasonal heat stress. The variation of IL-10 gene expression levels between the two breeds can be attributed to their geographical origins; the White Dorper emerging from arid subtropical South Africa and the Hungarian Indigenous Tsigai Sheep thriving in the seasonally harsh Carpathian climate for centuries.
Key words: gene expression / heat stress / Hungarian Tsigai / IL-10 / White Dorper
© The Authors, published by EDP Sciences, 2023
This is an Open Access article distributed under the terms of the Creative Commons Attribution License 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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