The effectiveness of cassava waste and skim milk as a filler of phytogenic and probiotic blends to inhibit the pathogenic bacteria and aflatoxin

¹ Master Student, Faculty of Animal Science, Universitas Brawijaya, Malang 65145, Indonesia
² Lecturer, Faculty of Animal Science, Universitas Brawijaya, Malang 65145, Indonesia 3Lecturer, Faculty of Veterinary Medicine, Universitas Brawijaya, Malang 65145, Indonesia

^* Corresponding author: emhanatsir@ub.ac.id

Abstract

This research aims to determine the effectiveness of cassava waste and skim milk as a filler of phytogenic and probiotic blends to inhibit phtytogenic bacteria and aflatoxin. The phytogenics and probiotics blends were used in the yellow type with the composition of Galangal (Kaempferia rhizome), Temulawak (Curcuma xanthorrihiza roxb), Red Ginger (Zingiber officinale), Turmeric (Curcuma domestica val), Actinomycetes, lactic acid bacteria, photosynthetic bacteria, tempeh yeast, and fermentation fungi (Aspergillus); furthermore, the green type consists of Sambiloto (Andrographis paniculata), Betel (Piper betle), Moringa (Moringa Oliefera), Papaya (Carica papaya), actinomycetes, lactic acid bacteria, photosynthetic bacteria, tempe yeast, and fermentation fungi (Aspergillus). Each pytobiotic and probiotic in yellow and green types were filled with cassava waste and skim milk, then dried in an oven at 50 °C for 24 hours. The ratio between the combination of phytogenics and probiotics with the filler is 1:1. The inhibition was divided into four types, consisting of positive control, negative control, cassava waste, and skim milk. The inhibitory pathogenic bacteria and fungi used the Well Method. The data were analyzed using a complete randomized design. If there were a significantly different result, then the analysis of the Duncan Multiple Range Test (DMRT) would be continued. The results showed that control positive in the yellow and green type has the higher inhibitory pathogenic on salmonella, escherichia coli, and aflatoxin. However, the filler casava waste has higher inhibitory salmonella, escherichia coli, and aflatoxin compared to skim milk. The conclusion of this research showed that cassava waste is effective as a filler for phytogenic and probiotics as an inhibitory pathogenic bacteria and aflatoxin.