BIO Web Conf.
Volume 85, 20243rd International Conference on Research of Agricultural and Food Technologies (I-CRAFT-2023)
|Number of page(s)
|Research of Agricultural and Food Technologies
|09 January 2024
Investigation of genotype and allele distribution of ppargc1a-t19c locus in holstein cattle
Cukurova University, Faculty of Agriculture, Department of Animal Science, Adana, Turkiye
* Corresponding author: email@example.com
This study aimed to investigate the genotype and allele distribution of the PPARGC1A-T19C locus in Holstein cattle. The PPARGC1A gene plays a crucial role in significant metabolic processes such as adaptive thermogenesis, gluconeogenesis, skeletal muscle fiber type switching, mitochondrial biogenesis, and adipogenesis. Additionally, it holds a key role in the activation of many hormone receptors and transcription factors, thereby emerging as a potential candidate gene for the milk traits of cattle. In this study, genotyping was performed using the PCR-RFLP method on 50 Holstein animal samples. The 205 bp PCR product was digested with the BsuRI restriction enzyme to reveal genotypic differentiation. Two different genotypes, TC and CC, were identified. The genotype frequencies were found to be 0.30 (TC) and 0.70 (CC). Allele frequencies were determined as 0.15 (T) and 0.85 (C). The Chi-square test showed that the PPARGC1A-T19C locus distribution was in Hardy-Weinberg equilibrium (p>0.05). In conclusion, these findings can be utilized to understand intra-breed genetic diversity and develop more effective selection strategies in the genetic improvement programs of the breed.
© The Authors, published by EDP Sciences, 2024
This is an Open Access article distributed under the terms of the Creative Commons Attribution License 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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