The activity of Pometia pinnata leaf extract against pathogenic bacteria in fish

¹ Department of Aquaculture, Faculty of Agriculture, Universitas Islam Riau, Pekanbaru 28284, Indonesia.
² Marine and Fisheries Faculty, Universitas Maritim Raja Ali Haji, Tanjungpinang, 19100, Indonesia

^* Corresponding author: jr.setiaji @agr.uir.ac.id

Abstract

The use of antibiotics to cure bacterial diseases has a negative impact on fish and the environment, so it is necessary to look for natural products that are safe for treating fish diseases. This study aims to determine the activity of P. pinnata extract as an antibacterial towards V. alginolyticus, P. aeruginosa, A. salmonicida, E. ictaluri, A. hydrophila, and E. tarda bacteria. P. pinnata extract was obtained by maceration using ethanol solvent. This extract was tested for its phytochemical content, characterized by FT-IR and tested for its antibacterial activity by the agar diffusion method. The results of the phytochemical test of P. pinnata extract produced terpenoids compounds flavonoids, saponins and phenolic. The results of the FT-IR characterization of P. pinnata extract contained O-H, C-H, C=O and C-O groups. The results of the inhibition test showed that P. pinnata extract was able to inhibit the growth of V. alginolyticus bacteria by 13.0 mm to 14.7 mm. P. aeruginosa 14.5 mm to 17.1 mm. A. salmonicida by 12.4 mm to 14.9 mm. E. ictaluri by 13.5 mm to 15.5 mm. A. hydrophila 13.9 mm to 17.1 mm. E. tarda 13.6 mm to 15.5 mm. In conclusion, P. pinnata extract can be used to inhibit the growth of pathogenic bacteria in fish.