| Issue |
BIO Web Conf.
Volume 87, 2024
The 5th International Conference on Fisheries, Aquatic, and Environmental Sciences (ICFAES 2023)
|
|
|---|---|---|
| Article Number | 03010 | |
| Number of page(s) | 6 | |
| Section | Fisheries (Biodiversity, Aquaculture, Food and Nutrition, Sustainable Seafood, Natural Resources, Conservation) | |
| DOI | https://doi.org/10.1051/bioconf/20248703010 | |
| Published online | 15 January 2024 | |
Climbing Perch Fish Sperm DNA (Anabas testudineus) Protection Using Cryoprotectant Agent
1 Graduate School of Mathematics and Applied Sciences, Universitas Syiah Kuala, Banda Aceh 23111, Indonesia
2 Department of Aquaculture, Faculty of Marine and Fisheries Sciences, Universitas Syiah Kuala, Banda Aceh 23111, Indonesia
3 Department of Marine Science, Faculty of Marine and Fisheries Sciences, Universitas Syiah Kuala, Banda Aceh 23111, Indonesia
4 Master Program of Biology, Faculty of Mathematics and Natural Sciences, Universitas Syiah Kuala, Banda Aceh 23111, Indonesia
* Corresponding author: muchlisinza@usk.ac.id
Sperm cryopreservation is a sperm storage method that needs to be developed to ensure the availability of climbing perch fish (Anabas testudineus) sperm considering the difficulty of providing quality broodstock. This study aims to determine the effect of several types and concentrations of cryoprotectants on the DNA integrity of climbing perch fish sperm after cryopreservation for 14 days at a temperature of -196 ℃. Analysis of sperm DNA integrity used the gel electrophoresis method. This research consists of four types of cryoprotectants; Each type of cryoprotectant consists of two concentration levels. The cryoprotectant concentrations used were DMSO (concentrations 10 and 20%), Methanol (concentrations 10 and 20%), Glycerol (concentrations 5 and 20%), and Ethanol (concentrations 5 and 15%) as well as control treatment and fresh sperm treatment. Based on the results of the DNA integrity analysis of climbing perch fish sperm, it showed that all sperm DNA samples did not form fragments, meaning that there was no damage to climbing perch fish sperm DNA in all samples tested, both fresh sperm samples and post-cryopreserved sperm samples.
© The Authors, published by EDP Sciences, 2024
This is an Open Access article distributed under the terms of the Creative Commons Attribution License 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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