Genetic Diversity Analysis of Exon-2 Growth Hormone (GH) Gene in Crossbred Chicken (Sentul X Arab) using PCR-RFLP Technique

¹ Faculty of Animal Science, Universitas Brawijaya, Malang 65145, Indonesia
² Faculty of Animal Science, Universitas Jambi, Jambi 36361, Indonesia

^* Corresponding author: suyadi@ub.ac.id

Abstract

The research aims to analyze the genetic diversity of the GH gene using the PCR-RFLP technique. Fifty blood samples were collected from crossed Sentul and Arab Chickens aged three months. DNA was extracted from whole blood samples, and its quantity was evaluated by bio-drop technique. Extracted DNA was amplified for gene GH using a PCR machine. The PCR product was restricted by MspI (C↓CGG) and Hae-III (GG↓CC) enzymes for identifying the fragment length diversity. The variables observed in this study were DNA quantitative analysis, the genotype, and alleles of the obtained genotype. The result shows the extracted DNA is of good quality because it has values >1.8 and <2.0, which means there’s no contamination in the DNA. GH gene was amplified with primers of 519 bp and showed that a DNA band matched the target in the specified primer design. Analysis of Genetic Diversity shows the monomorphic GH gene in this research. The genotype obtained at the GH-HaeIII locus is AA, and at the GH-MspI locus is TT. Both enzymes weren’t restricted to these amplification design sites.