Gene Expression Profiles of HSP70 and HSP90 Genes and Biochemical Responses in Juvenile Abalone, Haliotis diversicolor squamata in Response to Total Suspended Solids

¹ Research Center for Fishery, National Research and Innovation Agency (BRIN), Jl. Raya Bogor 16912, Cibinong, Bogor, Jawa Barat, Indonesia
² Research Center for Microbiology, National Research and Innovation Agency (BRIN), Jl. Raya Bogor 16912, Cibinong, Bogor, Jawa Barat, Indonesia
³ Department of Fisheries, Faculty of Agriculture, Universitas Gadjah Mada, Jl. Flora, 55281, Bulaksumur, Yogyakarta, Indonesia
⁴ Department of Genetics, Faculty of Biology, Gadjah Mada University, Jl. Flora, 55281, Bulaksumur, Yogyakarta, Indonesia

^* Corresponding author: sugengjokopurnomo70@gmail.com

Abstract

Prokaryotic and eukaryotic organisms contain heat shock proteins (HSPs), crucial for rapid response to environmental stress. However, their specific roles in different stress conditions are not fully understood. This study investigated HSP70 and HSP90 expression in H. diversicolor squamata, using qRT-PCR. Data analysis employed SPSS, including t-tests and ANOVA, with significance set at P < 0.05. Results showed distinct expression patterns of HSP genes under varying TSS levels. Both HSP70 and HSP90 mRNA levels significantly increased in response to TSS stressors, with HSP70 exhibiting the highest sensitivity to TSS changes. The duration and amount of TSS exposure influenced gene transcripts, particularly notable at 12 hours and 150 mgL-1 concentration. These findings suggest HSP genes play a role in cellular stress responses to environmental stimuli. HSP70 and HSP90, sensitive to TSS stress, can serve as biomarkers for assessing stress levels from TSS exposure and detecting TSS contamination in abalone farming.