| Issue |
BIO Web Conf.
Volume 94, 2024
The 8th International Conference on Biological Sciences “Leveraging Biodiversity to Support Green Economy and Climate Resilience” (ICBS 2023)
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|---|---|---|
| Article Number | 04011 | |
| Number of page(s) | 6 | |
| Section | Biosystematics, Ecology, and Evolution | |
| DOI | https://doi.org/10.1051/bioconf/20249404011 | |
| Published online | 25 March 2024 | |
Genetic differentiation of Hippa adactyla based on CO1 gene sequence in Sundaland
1 Animal Bioscience Study Program, Department of Biology, Faculty of Mathematics and Natural Sciences, IPB University, Kampus IPB Dramaga, Bogor, Indonesia.
2 Department of Aquatic Resources Management, Faculty of Fisheries and Marine Sciences, IPB University, Kampus IPB Dramaga, Bogor, Indonesia.
* Corresponding author: vendieko@apps.ipb.ac.id; achamad@apps.ipb.ac.id
The Hippidae is a family of sand crabs that inhabit intertidal ecosystems, specifically in sandy areas scattered throughout the coast. Differences in habitat conditions cause variations in Hippidae members. Hippa adactyla is a member of the genus Hippa. This study aimed to map the genetic differentiation between Hippa adactyla populations in the Greater Sunda Islands. The sand crab (Hippa adactyla) samples were taken from Sumatra (Pariaman, Bengkulu, Lampung) and Java (Pangandaran, Pelabuhan Ratu, Banyuwangi) by handcatching then stored in alcohol 96%. Total genomic DNA was extracted from the muscle tissue of the samples using a commercial DNA Extraction KIT. The upper part of the COI gene (5' end) (±750 bp) was amplified using specific primers. Based on the K2P substitution model, the genetic distance of the Pangandaran population to the Bali Strait population is the same as that of the Sumatran population (0.062). Genetic diversity ranges from 0.000 to 0.123. Haplotype diversity was 0.928, consisting of 18 haplotypes.
© The Authors, published by EDP Sciences, 2024
This is an Open Access article distributed under the terms of the Creative Commons Attribution License 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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