Effects of ultrasound waves on rat liver mitochondria

¹ University Alfraganus, Tashkent, 100190, Uzbekistan
² Tashkent State Pedagogical University named after Nizomi, Tashkent, 100185, Uzbekistan
³ Tashkent State Agrarian University, Tashkent, 100140, Uzbekistan
⁴ Chirchik State Pedagogical University, Tashkent, 110700, Uzbekistan

^* Corresponding author: d.babaxanova@afu.uz

Abstract

Succinate dehydrogenase has a wide range of properties in plant and animal cells and is distributed throughout the inner membrane of mitochondria, therefore it is the enzyme without which energy metabolism cannot occur. The goal of our research was to investigate the effect of ultrasonic waves on the intensity of lipid peroxidation, activity of succinate dehydrogenase and cytochrome c oxidase in rat liver mitochondria, as well as antioxidant effect of mulberry leaf extract and biosep oil extract. Study involved white lab rats weighing 180-200 grams. The ultrasound device used was Mindrey DP-50 Vet. The work was based on the following methods: Schneider differential centrifugation, spectrophotometry, chromatography, pH-metry, photometry. The results of the study explain the elucidation of the mechanisms of damage to liver tissue by ultrasonic waves and the correction of the mitochondrial dysfunction caused by it with the extract of mulberry leaves and the oil extract of biosep. It was revealed that changes in lipid peroxidation led to disruption of the functional activity of membrane-bound cytochrome c oxidase and succinate dehydrogenase.