Prediction of microRNA target genes for diagnosis and prognosis of asthma development

Al-Farabi Kazakh National University, Almaty, 050040, Kazakhstan

^* Corresponding author: Shara.Atambaeva@kaznu.kz

Abstract

Given the intricacy of diagnosis, there's an urgent necessity to establish objective and quantifiable biological indicators to enhance the accuracy of diagnosing asthma in its early stages. MicroRNAs emerge as promising candidates for asthma diagnosis. By leveraging the correlations between established target genes and microRNAs, a methodology for early asthma diagnosis can be formulated. A comprehensive database containing microRNAs and target genes implicated in asthma development has been compiled. The dynamics of microRNA interaction with target genes crucial for diagnosing and predicting the progression of asthma have been investigated. Specific microRNA binding sites with target genes involved in asthma development have been pinpointed. Using the DIANA-microTCDS program, features of microRNA interaction with target genes linked to asthma development have been analyzed. Notably, binding sites with significant occurrences have been identified in genes such as IL13, VEGFA, ADRB2, ALDH2, IL10, and DPP10. The conservativity of microRNA binding sites to mRNA genes has been demonstrated.