Isolation and selection of nodulating bacteria from the rhizosphere of legume (mash - Vigna radiata) and bean (Phaseolus lunatus) plants and to enhance specific properties through induced mutagenesis methods

Al-Farabi Kazakh National University, Almaty 050040, Kazakhstan

Abstract

The aim of the research is to create a collection of PGPR properties of bacteria for legume plants, as well as to increase their growth—stimulating activity by methods of induced mutagenesis. In this article, studies have been conducted on the isolation of microorganisms from the rhizosphere of legumes. And also, to increase the PGPR growth-stimulating properties of rhizosphere microorganisms using methods of physical and chemical mutagenesis. A collection of active rhizosphere bacteria has been created from 4 strains of the genus Bacillus megaterium, Arthrobacter globiformis, Arthrobacter crystallopoietes and Bacillus spp. Dining the screening, bacteria with PGPR properties Bacillus megaterium, Arthrobacter globiformis, and Arthrobacter crystallopoietes were isolated, as these strains stimulated the growth of legume plants.