| Issue |
BIO Web Conf.
Volume 100, 2024
International Scientific Forum “Modern Trends in Sustainable Development of Biological Sciences” (IFBioScFU 2024)
|
|
|---|---|---|
| Article Number | 03003 | |
| Number of page(s) | 7 | |
| Section | Fundamental and Applied Research in Genetics and Molecular Biology | |
| DOI | https://doi.org/10.1051/bioconf/202410003003 | |
| Published online | 08 April 2024 | |
Application of the switchSENSE® technology for real-time study of pesticides interaction with biological molecules
1 Université de Lorraine, INRAE, L2A, F-54000 Nancy, France
2 Institute of Genetics and Physiology, Almaty, 050060, Kazakhstan
3 al-Farabi Kazakh National University, Almaty, 050040, Kazakhstan
4 Université de Lorraine, INRAE, IAM, F-54000 Nancy, France
* Corresponding author: baratzhanova.gulminyam@gmail.com
Organochlorine pesticides have been extensively utilized in agriculture and pest control, and still contributing to numerous health issues. However, the mechanism underlying the transportation of these compounds through animal and human body is not well understood. The switchSENSE® technology is an original and powerful tool in biosensing, which demonstrates high sensitivity in detecting a variety of biological interaction which involves proteins, nucleic acids and small molecules. There has been a growing interest in using switchSENSE® technology for detecting interactions between proteins and environmental pollutants in recent years. Therefore, the aim of this study is to refine and enhance the methodology of the switchSENSE® technology to facilitate characterization of real time interaction between biological transport molecule, bovine serum albumin (BSA), and organochlorine pesticides. Using this technology, we noticed the conformational change in structure and protein hydrodynamic diameter (DH) of BSA in response to Chlordecone (CLD) and Dichlorodiphenyltrichloroethane (DDT). We also identified the possible obstacles, that should be resolved in future researches.
© The Authors, published by EDP Sciences, 2024
This is an Open Access article distributed under the terms of the Creative Commons Attribution License 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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