| Issue |
BIO Web Conf.
Volume 101, 2024
The 5th International Conference on Life Sciences and Biotechnology (ICOLIB 2023)
|
|
|---|---|---|
| Article Number | 01002 | |
| Number of page(s) | 10 | |
| Section | Applied Science | |
| DOI | https://doi.org/10.1051/bioconf/202410101002 | |
| Published online | 09 April 2024 | |
Coffee Pulp Activated Carbon for Immobilizing Cellulase from Aspergillus niger ICP2: Enhancing Enzyme Stability, Activity, and Its Reusability
1 Department of Biology, Faculty of Mathematics and Natural Sciences, Jember University, Jalan Kalimantan 37 Kampus Tegal Boto 68121 Jember, Indonesia
2 Department of Chemistry, Faculty of Mathematics and Natural Sciences, Jember University, Jalan Kalimantan 37 Kampus Tegal Boto 68121 Jember, Indonesia
* Corresponding author: kaharmzk@unej.ac.id
Cellulase from Aspergillus niger ICP2 was successfully immobilized on coffee pulp activated carbon using adsorption. Carbon was derived from coffee pulp via controlled carbonization at 200 °C for 2 hours, followed by activation with 3M ZnCl2 for 150 minutes. Reusability analysis exhibited over 50% relative activity retention after four cycles. The immobilized cellulase demonstrated stability at pH 4.0-6.0, retaining over 80% relative activity after 4 hours at 37℃. This approach proves economical for enhancing cellulase stability, activity, and reusability.
© The Authors, published by EDP Sciences, 2024
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