Phenotyping of experimental rice population in accelerated breeding of new varieties with valuable grain quality traits

1Federal Scientific Rice Centre, 350921 Belozerny, Russia

^* Corresponding author: tngerag@yandex.ru

Abstract

The study of the consumer advantages of rice samples was carried out in connection with the development of an effective breeding methodology for developing rice genotypes based on an accelerated breeding process. The aim of the research was to study the protein and amylose content in rice grain samples of the parent white-grain and red-grain forms and generations of BC₂. In the program of developing varieties based on the use of modern postgenomic and cellular technologies, BC₂ and their parent forms - samples of the USI “Collection of the FSC of rice” belonging to various groups by the grain form, white-grained, served as the research material. The crop was grown on the experimental plot of Federal Scientific Rice Centre. As a result of the work carried out, samples with an unpainted pericarp were isolated for large-scale genotyping. Phenotyping of the studied rice samples was carried out by protein and amylose content in rice grains. The assessment on the basis of “protein content” was carried out using a universal infrared express analyzer “Multicheck Plus”, InfraLUM FT 6, the amylose content in starch of rice grain varieties – according to GOST ISO 6647-2-2015 Fig. BC₂ groups with a high protein content, more than 8.8% and amylose content 1 – 16.0-19.0 %, 2 - amylose content 20.0-22.0%, 3 - amylose content 22.1-27.0% were identified in BC₂ grain combinations.