| Issue |
BIO Web Conf.
Volume 126, 2024
International Conference on Advance in Energy, Ecology and Agriculture (AEEA2024)
|
|
|---|---|---|
| Article Number | 01049 | |
| Number of page(s) | 7 | |
| DOI | https://doi.org/10.1051/bioconf/202412601049 | |
| Published online | 28 August 2024 | |
Study of the microbiome associated with stem crown galls of grapes
Kuban State Agrarian University, Center for Biotechnology, Krasnodar, 350044, Russia
* Corresponding author: kopylcov.s@edu.kubsau.ru
The work investigated the species composition of the bacterial microbiome associated with stem crown galls on the grape variety Kishmish radiant (Cardinal x Kishmish pink). The result of the identification of bacterial isolates using MALDI-TOF mass spectrometry, molecular genetic analysis, and the cultural method on nutrient media made it possible to identify them in stem crown galls of grapes is presented. The following microorganisms were identified: Pseudomonas koreensis, Roaultella planticola, Enterobacter cloacae, Pseudomonas fulva, Xanthomonas bonasiae, Winslowiella toletana. Using a biological test on carrots, the pathogenicity of two species Xanthomonas bonasiae, Winslowiella toletana was determined by the ability to form galls, while no virulence genes were detected by PCR analysis using specific primer pairs of genes.
© The Authors, published by EDP Sciences, 2024
This is an Open Access article distributed under the terms of the Creative Commons Attribution License 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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