| Issue |
BIO Web Conf.
Volume 127, 2024
The International Conference and Workshop on Biotechnology (ICW Biotech 2024)
|
|
|---|---|---|
| Article Number | 01009 | |
| Number of page(s) | 7 | |
| Section | Agricultural Biotechnology for Food Improvement and Production | |
| DOI | https://doi.org/10.1051/bioconf/202412701009 | |
| Published online | 13 September 2024 | |
Extended spectrum β-lactamase (ESBL) OF Escherichia coli resistance from the broiler cloaca in the traditional market banda aceh
1 Laboratory of Veterinary Public Health, Faculty of Veterinary Medicine, Universitas Syiah Kuala, Banda Aceh, Indonesia.
2 Laboratory of Veterinary Histology, Faculty of Veterinary Medicine, Universitas Syiah Kuala, Aceh, Indonesia.
3 Undergraduate Student in Faculty of Veterinary Medicine Universitas Syiah Kuala, Banda Aceh, Indonesia.
* Corresponding author: rastina_rzl@usk.ac.id
Extended Spectrum β-Lactamase (ESBL)-producing Escherichia coli is a type of bacteria that can cause several infectious diseases in various animal and human tissues. ESBL bacteria have been reported to be resistant to various classes of antibiotics including penicillin, third-generation cephalosporins (cefotaxime, ceftriaxone and ceftazidime), and monobactam (aztreonam). This study focused on the presence of Escherichia coli as an ESBL producer in the cloaca of broiler. The purpose of this study was to determine the presence or absence of ESBL-producing Escherichia coli in the cloaca of broiler in the traditional market, Banda Aceh. This study used 47 samples of broiler cloacal swabs. The study used Cross-Sectional Observation and the disc diffusion methods. The presence of ESBL-producing Escherichia coli in cloacal swabs was identified using Eosin Methylene Blue Agar (EMBA) media. Detection of ESBL production using β-lactam antibiotic discs, namely cefotaxime, ceftazidime and ampicillin. The results showed the presence of ESBL-producing Escherichia coli bacteria isolated from cloacal swabs of broiler in the traditional market, Banda Aceh, which reached 21.27% (10/47). Sensitivity test results showed high resistance of Escherichia coli to cefotaxime 100% (10/10), ceftazidime 90% (9/10), and ampicillin 70% (7/10). Referring to CLSI, all isolates (100%) were ESBL-producing Escherichia coli. Based on the results of the study, it can be concluded that there is Extended Spectrum β-lactamase (ESBL) producing Escherichia coli in the cloaca of broiler in the traditional market, Banda Aceh.
© The Authors, published by EDP Sciences, 2024
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