Identification of histon H2A protein and amino acid profiles gonggong sea snails (Leavistrombus turturella) as antimicrobial peptides candidate

¹ Department of Aquatic Product Technology, Raja Ali Haji Maritime University, Riau Islands, Indonesia
² South East Asian Food and Agricultural Science and Technology (SEAFAST) Center, IPB University, West Java, Indonesia
³ Halal Study Center and Halal Inspection of Raja Ali Haji Maritime University, Riau Islands, Indonesia
⁴ Department of Aquaculture, Raja Ali Haji Maritime University, Riau Islands, Indonesia
⁵ Department of Aquatic Product Technology, IPB University, West Java, Indonesia

^* Corresponding author: lilyviruly@umrah.ac.id

Abstract

Previous study showed that Gonggong sea snail (GSS) meat extracts as natural antibiotic inhibited Escherichia coli and Staphylococcus aureus, but the type of protein is not yet known. The characteristic antimicrobial peptides (AMPs) of gastropods are generally the histon H2A protein, which is consist of cationic (lysine, arginine) and hydrophobic (leucine, proline, alanine, phenylalanine, and valine) amino acids. This research aimed to explore of potency of GSS meat extract as alternative source of AMPs via amino acid profiles and identification of phylogenetic trees of the histone H2A proteins. The amino acid profiles were analysed using the HPLC. The phylogenetic tree of the histone H2A proteins were analyzed using the MEGA X. The histone H2A proteins of GSS were clustered with the histone H2A proteins from the other gastropods. The histon H2A protein of GSS had 80% similarity with the clade of Molluskin (histon H2A protein of Fiscus gracilis). They had 19-20 amino acids and were included in histone H2A protein as AMPs. The amino acid profiles of GSS had cationic, anionic and hydrophobic amino acid. These results can be used as the first information for developing AMPs from GSS meat extract in the field of natural preservative exploration and pharmaceutical.