Issue |
BIO Web Conf.
Volume 136, 2024
The 13th International and National Seminar of Fisheries and Marine Science (ISFM XIII 2024)
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Article Number | 04002 | |
Number of page(s) | 17 | |
Section | Marine, Fisheries Biotechnology, Ocenography, and Fishing Technology | |
DOI | https://doi.org/10.1051/bioconf/202413604002 | |
Published online | 11 November 2024 |
Chemical Profile, Antioxidant and Cytotoxic Potencies and Docking Study of Semipolar Fraction of Nepthea sp.
1 Faculty of Pharmacy, Universitas Halu Oleo, Kendari, Indonesia
2 Faculty of Marine Sciences and Fisheries, Universitas Halu oleo, Kendari, Indonesia
3 Faculty of Medicine, Universitas Halu Oleo, Kendari, Indonesia
4 Health Polytechnics of Bina Husada, Kendari, Indonesia
5 Research Centre for Chemistry, National Research and Inovation Agency (BRIN), Kawasan Puspitek, Tangerang Selatan 15314, Banten, Indonesia
* Corresponding author: sahidin02@uho.ac.id
The aims of research is to explore pharmaceutical and chemicals aspects of Nepthea sp. The methods include fractionation of ethylacetate extract (EAE) by vacuum liquid chromatrography (VLC). The chemical content was evaluated by LC-MS/MS analysis, phytochemical screening, Total Phenolics Content (TPC) and Total Flavonoids Contents (TFC). Antioxidant and cytotoxic potencies were evaluated by DPPH/ABTS methods and MTT assays and also docking study. Fractionation of EAE produced six fractions (1-6). Fraction 3 and 4 were semi-polar compounds, which contained terpenoids, phenolics and alkaloids. According to LCMS/MS, the identified semi-polar compounds consist of 3-acetyl-3,4-dihydro-5,6-dimethoxy-2(1)H-benzopyrone and oxyphyllenone B from Fraction 3 and 1-(4-hydroxy-3-methoxy)-phenyl-1,2,3-propanetriol, digiprolactone, petasitolone, δ-humulene, rengyolester, and piperolactam-C9:1(8E) for Fraction 4. The antioxidant potency of fraction 4 was better than Fraction 3. Based on IC50 value, Fraction 3 is active category and Fraction 4 is moderately active against MCF-7 cell lines. At the molecular level, rengyolester and piperolactam-C9:1(8E) revealed potential antioxidant activity by inhibiting XO, which was correlated with antiradical in vitro results. Meanwhile, the anticancer activity of oxyphyllenone B and piperolactam-C9:1(8E) demonstrated a higher affinity for inhibiting ERα than other compounds. As conclusion, semipolar fractions of EAE have good potential as an antioxidant and cytotoxic agent against MCF-7 cell lines.
© The Authors, published by EDP Sciences, 2024
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