| Issue |
BIO Web Conf.
Volume 139, 2024
International Scientific and Practical Conference “AGRONOMY – 2024” (AgriScience2024)
|
|
|---|---|---|
| Article Number | 01008 | |
| Number of page(s) | 8 | |
| Section | General Farming and Crop Production | |
| DOI | https://doi.org/10.1051/bioconf/202413901008 | |
| Published online | 15 November 2024 | |
Prospects for obtaining planting material of a less widespread berry crop – black crowberry (Empetrum nigrum L.) in vitro conditions
1 Russian State Agrarian University – Moscow Timiryazev Agricultural Academy, Timiryazevskaya street, 49, 127434, Moscow, Russia
2 All-Russian Phytopathology Research Institute, St. Institute, 143050, Big Vyazyomy, Russia
* Corresponding author: vannechiporenko@gmail.com
This study was conducted to evaluate the morphological and morphometric characteristics of a sparsely distributed berry crop – Empetrum nigrum L. cultivar Irland in 2023-2024 during in vitro culture initiation and multiplication stages. The results showed that the best growth-stimulating hormone for sterile culture initiation is – 6-BAP at a concentration of 0.2 μM, at which the grafting rate of explants was 73.3%. As a result of the studies, at the multiplication stage, the advantage of using Zeatin (2.2 μM) as a growth-stimulating hormone when using agar-agar AT (American type) as a gel-forming agent was revealed, and the productivity of microshoots was 3.68 times higher than that of the control.
© The Authors, published by EDP Sciences, 2024
This is an Open Access article distributed under the terms of the Creative Commons Attribution License 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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