Metallic nanoparticles production by Actinomycetes and their Impact against some human pathogens

¹ Botany and Microbiology Department, Faculty of Science, Cairo University, Cairo, Egypt
² Biology Department, Darb University College, Jazan University, Jazan, Saudi Arabia
³ Biophysics Department, Faculty of Science, Cairo University, Cairo, Egypt
⁴ Clinical Pathology Department, Faculty of Medicine, Cairo University, Cairo, Egypt
⁵ Department of Microbiology, Egyptian Drug Authority, 11511, Cairo, Egypt

^* Corresponding author: safya.ahmed1993@gmail.com

Abstract

The Study Investigated Metallic Nanoparticles Synthesized extracellularly. Using actinomycetes isolated from different environments as (The Mediterranean Sea, Some Fields, stagnant water, underground water, and rainfall water). Checking for Microorganisms Only six actinomycetes could be retrieved from the thirty-five samples that were gathered for the biosynthesis of metallic nanoparticles, and of those six, only three showed the ability to synthesis AgNPs with antibacterial activity. We identified these powerful isolates as Arthrobacter crystallopoietes strain smmdk12 (OR685674), Micromonospora maris strain smmdk13 (OR685672), and Streptomyces albus strain smmdk14 (OR685674) based on their features. On the basis of 16rRNA sequences along with morphological and physiological properties. The proliferation of medically significant pathogenic bacteria was considerably influenced and suppressed by the biosynthesized AgNPS. Silver nitrate (1 mM) was added to the culture supernatant to create biosynthesized AgNPS, which were then characterised using transmission electron microscopy, Fourier transform infrared spectroscopy, and ultraviolet visible spectrophotometer. The AgNPS particle size and spherical shape that were obtained (6.46nm-24.7nm). Evaluations were conducted on the antimicrobial impact of AgNPs. The results of the wound healing process showed that after being exposed to Ag-NPs for 24 hours, fibroblasts progressed toward the opening to seal the scratch wound by around 66.4%, significantly speeding up the healing process.