Intensification of the process of ergosterol accumulation by yeast Saccharomyces cerevisiae by changing the composition of the culture medium and using ultrasound

¹ ITMO University, Kronverksky Prospekt, 49, lit. A, 197101 St. Petersburg, Russian Federation
² South Ural State University (National Research University), 76 Lenina Avenue, 454080 Chelyabinsk, Russian Federation

^* Corresponding author: ds-ilenka@mail.ru

Abstract

The chemical synthesis of vitamin D2, an important nutrient for living organisms, is complex due to the asymmetric structure of the vitamin molecule. This opens up significant prospects for the development of biotechnological approaches to cyanocobalamin synthesis, which forms the basis of this work. The study considers the possibility of using nutrient media of different compositions, as well as ultrasound exposure to intensify the biosynthesis of ergosterol by yeast Saccharomyces cerevisiae as a precursor of vitamin D2. The standard YEPD medium is considered as a nutrient medium, as well as low-carb and sodium nitrate dilution. The modes of ultrasonic exposure varied in power and processing time, 9 variants were evaluated. Together, the intensification of the process of ergosterol biosynthesis by yeast cells can ensure the economic viability, stability, and expediency of its production on an industrial scale. The research results have shown the expediency of the proposed approaches. Thus, the introduction of sodium nitrate into the nutrient medium allowed for an increase in synthesized ergosterol in the yeast biomass by 18.2% compared with the standard medium. Optimization of ultrasonic treatment modes has shown the possibility of achieving the synthesis of ergosterol to a content of 11.82% per dry matter.