| Issue |
BIO Web Conf.
Volume 142, 2024
2024 International Symposium on Agricultural Engineering and Biology (ISAEB 2024)
|
|
|---|---|---|
| Article Number | 03010 | |
| Number of page(s) | 7 | |
| Section | Microbial Engineering and Biomedical Exploration | |
| DOI | https://doi.org/10.1051/bioconf/202414203010 | |
| Published online | 21 November 2024 | |
Development of a colloidal gold immunoassay based on monoclonal antibody for tomato spotted wilt disease
1 Yunnan Tobacco Quality Supervision and Inspection Station, Kunming, Yunnan 650106, China
2 Kunming Cigarette Factory of Hongyun Honghe Group, Kunming, Yunnan, 650506, China
* awangchunqiong.ytqs@outlook.com; b*13888092288@139.com; c* chen.dana@qq.com
Tomato spotted wilt virus (TSWV) is a bacterial virus that seriously harms the growth of tobacco. In order to realize the rapid detection of TSWV, monoclonal antibody to TSWV-NP protein was prepared and characterized, and colloidal gold immunochromatographic test strips were established that can be used to detect TSWV. In this study, the monoclonal antibody to TSWV-NP protein was labeled with colloidal gold, and the reaction conditions were optimized to establish a colloidal gold immunochromatographic test strip assay, and the detectability, accuracy, and specificity of the test strip were evaluated. The detection limit of the test strip for TSWV-NP protein was 0.1 μg/mL, and there was no cross-reactivity with tobacco bunchy top virus, potato Y virus, and tobacco mosaic virus, which had excellent specificity. The results of the colloidal gold test strip and the RT-PCR result method were compared with the actual samples, and the total conformity rate of the two was 95%, and the positive conformity rate was 93.3%.
© The Authors, published by EDP Sciences, 2024
This is an Open Access article distributed under the terms of the Creative Commons Attribution License 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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