Catalase Biomimetic Sensor Based on Metal

¹ Nagiev Institute of Catalysis and Inorganic Chemistry of Ministry of Science and Education, Baku, Azerbaijan
² Western Caspian University, Baku, Azerbaijan
³ Vice-president of Azerbaijan National Academy of Sciences, Director of Research Center of “Azerbaijan National Encyclopedia” and Department chief of Nagiev Institute of Catalysis and Inorganic Chemistry, Baku, Azerbaijan
⁴ Samarkand State Medical University, Samarkand, Uzbekistan
⁵ Oriental University, Tashkent, Uzbekistan

^* Corresponding author: rrio80@mail.ru

Abstract

The development of motivated biosensors associated with the study of a large number of different substances is one of the most important areas of development of analytical chemistry. This goal in the development of certain biosensors was necessary for a sufficiently clear, fast, qualitative and quantitative determination of the desired component. Chemical reactions in living organisms are carried out with the help of catalysts - enzymes. Enzymes are proteins with prosthetic groups, and proteins are polymers formed from amino acid links. The number of different enzymes in a cell is the same as the number of different chemical reactions that occur in it. This work is devoted to the study of biomimetic sensors, where metals (Pb, Ag, Al) were used as a converter. The active material was iron tetraphenylporphyrin adsorbed on Al₂O₃. It was found that biomimetic sensors made on the basis of intelligent material (TPhPFe3+/Al2O3) and metals have a number of technological advantages. The developed biomimetic sensors are characterized by high sensitivity, activity, stability and reproducibility. When detecting the catalase activity of biomimetic sensors, the maximum sensitivity to the concentration of H₂O₂ in an aqueous solution for TPhPFe³⁺/Al₂O₃//Pb was 10^-8wt.%, for TPhPFe³⁺/Al₂O₃//Ag – 10^-8wt.%, for TPhPFe³⁺/Al₂O₃//Al – 10^-6 wt.%.