Issue |
BIO Web Conf.
Volume 152, 2025
International Conference on Health and Biological Science (ICHBS 2024)
|
|
---|---|---|
Article Number | 01029 | |
Number of page(s) | 10 | |
Section | Dense Matter | |
DOI | https://doi.org/10.1051/bioconf/202515201029 | |
Published online | 20 January 2025 |
Analysis of microorganisms and antibiotic resistance in medical instruments in the central sterile supply department (CSSD) of hospitals before and after sterilization
1 Doctoral Program of Environmental Science, Post Graduate School of Universitas Sebelas Maret, Indonesia
2 Departement of Biology Education, Faculty of teacher and education Universitas Sebelas Maret, Indonesia
3 Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Sebelas Maret, Indonesia
4 Faculty of Medicine Universitas Sebelas Maret, Indonesia
* Corresponding author: worohapsari0@gmail.com
Sterilization is essential in preventing the spread of pathogenic bacteria and antibiotic resistance in hospital settings. This study examines the microbial growth and antibiotic resistance of seven medical instruments from the Central Supply Sterile Department, before and after sterilization. The instruments analyzed include arterial clamps, anatomical forceps, tissue scissors, surgical forceps, nal puder, open small tray, and instrument tray. The methodology involved in vitro microbiological testing, including Gram staining, colony counting, and the Vitek 2 system for bacterial identification and antibiotic susceptibility testing (AST). Results showed that before sterilization, nal puder was contaminated with Staphylococcus hominis ssp hominis (Gram-positive) and Bacillus sp. (Gram-positive), while the instrument tray was contaminated with Pseudomonas stutzeri (Gramnegative). After sterilization and a 90-day storage period, Staphylococcus hominis ssp hominis was detected on the open small tray. AST revealed an increase in bacterial sensitivity to antibiotics, from 12 to 14 types poststerilization. The minimum inhibitory concentration (MIC) for Benzylpenicillin decreased from 0.25 μg/mL (resistant) to ≤0.03 μg/mL (sensitive). This study highlights the critical role of sterilization in infection control and antibiotic resistance management in healthcare settings.
© The Authors, published by EDP Sciences, 2025
This is an Open Access article distributed under the terms of the Creative Commons Attribution License 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Current usage metrics show cumulative count of Article Views (full-text article views including HTML views, PDF and ePub downloads, according to the available data) and Abstracts Views on Vision4Press platform.
Data correspond to usage on the plateform after 2015. The current usage metrics is available 48-96 hours after online publication and is updated daily on week days.
Initial download of the metrics may take a while.