Issue |
BIO Web Conf.
Volume 154, 2025
15th International Conference on Global Resource Conservation (ICGRC 2024) in conjunction with the 1st International Conference on Jamu and Alternative Medicine (ICJAM 2024)
|
|
---|---|---|
Article Number | 01005 | |
Number of page(s) | 8 | |
Section | Biodiversity and Conservation | |
DOI | https://doi.org/10.1051/bioconf/202515401005 | |
Published online | 28 January 2025 |
The effect of water content in soil media on the hatchability of Bractocera dorsalis pupae
1 Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Brawijaya, Malang 65145, Indonesia
2 Department of Soil Science, Faculty of Agriculture, Universitas Brawijaya, Malang 65145, Indonesia
* Corresponding author: amin28@ub.ac.id
Fruit flies are polyphagous pests on various important fruit crops. The Bactrocera genus, including Bactrocera dorsalis (Hendel), undergoes a pupation process in the soil. Efforts to control this species in the pupal phase can be done by applying entomopathogenic fungi. Entomopathogenic fungi need a moist environment to grow optimally. This study aims to evaluate the effect of adding water to soil media on the success of B. dorsalis pupae becoming imago. The soil media used comes from orange fields. Soil media is sterilized first before being used to test the success of fruit fly pupation. The addition of water was carried out with the following water and media content ratios: 0 : 1, 0.075 : 1, 0.15 : 1, 0.184 : 1, 0.225 : 1, 0.3 : 1 and 0.5 :1. The pupa is placed in a mica tube with a diameter of 5 cm and poured with granulated soil, so that it reaches a height of 4 cm. Replication was carried out four times. The parameters observed include success in becoming an imago, survival, and normality. The highest percentage of pupae that hatched was found at a water to soil addition ratio of 0.15 to 1 (86.25% ± 5.54). The highest percentage of living imago was also at the same water addition concentration (27.5% ± 1.44), as was the percentage of normal imago (81.25% ± 5.90). However, the death rate at this concentration was also high (58.75& ± 4.73). The highest percentage of abnormal pupae was found in media without the addition of water (0: 1). There was a tendency for success rates to become imago, survival, and normality to decrease at higher water concentrations. The findings of the regression analysis show the survival equation y = -4.33x + 28.75. No pupae successfully hatched at a water concentration of 0.5 to 1 v/v. At concentration after two days, the soil becomes dry and hard to clot. This study recommends using a water concentration of 0.15 to 1 v/v for applications with entomopathogenic fungi to create sufficient soil moisture.
© The Authors, published by EDP Sciences, 2025
This is an Open Access article distributed under the terms of the Creative Commons Attribution License 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Current usage metrics show cumulative count of Article Views (full-text article views including HTML views, PDF and ePub downloads, according to the available data) and Abstracts Views on Vision4Press platform.
Data correspond to usage on the plateform after 2015. The current usage metrics is available 48-96 hours after online publication and is updated daily on week days.
Initial download of the metrics may take a while.