Issue |
BIO Web Conf.
Volume 154, 2025
15th International Conference on Global Resource Conservation (ICGRC 2024) in conjunction with the 1st International Conference on Jamu and Alternative Medicine (ICJAM 2024)
|
|
---|---|---|
Article Number | 03011 | |
Number of page(s) | 9 | |
Section | Jamu and Alternative Medicine | |
DOI | https://doi.org/10.1051/bioconf/202515403011 | |
Published online | 28 January 2025 |
Acute toxicity test and lethal dose (LD50) from a combination of sambiloto extracts (Andrographis paniculate) and catfish oil (Pangasius micronema Blkr.) in mice
Department of Pharmacy, Vocational School, Universitas Sebelas Maret, Surakarta, Indonesia
* Corresponding author: heru_sasongko@staff.uns.ac.id
Sambiloto (Andrographis paniculata) contains andrographolide compounds, which have anti-inflammatory properties. On the other hand, catfish oil (Pangasius micronema Blkr.) contains DHA and EPA compounds known to be unsaturated fatty acids and have antiinflammatory properties. Sambiloto extracts and catfish oil will be combined to determine the pharmacological effects caused by an acute toxicity test to determine the toxicity and LD50 value of the combination of extracts. This research is conducted experimentally, utilizing mice as test animals. The extract was administered for 14 days, and symptoms of toxicity were observed, including the number of animals that died, body weight, and the weight of the liver, kidneys, and heart. The data was then tested using the Thompson-Weil method and statistical tests using the oneway ANOVA test and continued with the Tukey post hoc test. The toxicity test results indicated toxicity symptoms at the highest dose, but the combination of extracts’ LD50 value was pseudo-LD50, implying that test animals could theoretically still receive the highest dose value. The statistical analysis revealed no significant differences in the body and organ weight of the mice, indicating that this research could still use the combination of the two extracts up to the highest dose.
© The Authors, published by EDP Sciences, 2025
This is an Open Access article distributed under the terms of the Creative Commons Attribution License 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Current usage metrics show cumulative count of Article Views (full-text article views including HTML views, PDF and ePub downloads, according to the available data) and Abstracts Views on Vision4Press platform.
Data correspond to usage on the plateform after 2015. The current usage metrics is available 48-96 hours after online publication and is updated daily on week days.
Initial download of the metrics may take a while.