Issue |
BIO Web Conf.
Volume 155, 2025
10th-ICCC – 10th International Conference on Climate Change “Climate Change, Plant and Health”
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Article Number | 10006 | |
Number of page(s) | 6 | |
Section | Climate Change Impact on Health and Adaptation/Mitigation Strategy | |
DOI | https://doi.org/10.1051/bioconf/202515510006 | |
Published online | 29 January 2025 |
Direct PCR for DNA barcoding of Bulbophyllum lobbii Lindl. based on rbcL sequence
Biology Department, Faculty of Mathematics and Natural Sciences, University of Jember, Jember 68121, Indonesia
* Corresponding author: msuudi.fmipa@unej.ac.id
DNA barcoding is a molecular technique frequently used to identify or confirm a species, which involves the steps of isolation, amplification via PCR, and sequencing analysis. However, the use of lysate derived from samples after soaked and heated in TE buffer is lacking reported for this application. This study aims to provide an alternative method for PCR using lysate as template for species identification of Bulbophyllum lobbii using rbcL primers. The results show that the lysate (after heating and briefly spun) is worthy of use as a template in PCR amplification, able to produce a thick single band with appropriate amplicon size (±600 bp). Further sequencing analysis confirms that the resulting sequence is highly readable with a clear chromatogram. BLAST analysis shows high identity (100%) with Bulbophyllum lobbii (MT518983) from USA. In sum, direct PCR using lysate provides an alternative approach for rapid DNA barcoding of plant samples with promising results.
© The Authors, published by EDP Sciences, 2025
This is an Open Access article distributed under the terms of the Creative Commons Attribution License 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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