Issue |
BIO Web Conf.
Volume 156, 2025
The 6th International Conference on Fisheries, Aquatic, and Environmental Sciences (ICFAES 2024)
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Article Number | 03027 | |
Number of page(s) | 9 | |
Section | Fisheries (Biodiversity, Aquaculture, Food and Nutrition, Sustainable Seafood, Natural Resources, Conservation, Capture Fisheries) | |
DOI | https://doi.org/10.1051/bioconf/202515603027 | |
Published online | 30 January 2025 |
Addition of garlic (Allium sativum) extract in fry as immunostimulant on survival and blood profile of pomfret fish (Colossoma macropomum)
Aquaculture Department, Faculty of Marine and Fisheries, Universitas Syiah Kuala, Banda Aceh, 23111, Indonesia
* Corresponding author: Ismarica@usk.ac.id
Pomfret fish (Colossoma macropomum) is a highly valuable freshwater fishery commodity. Bacterial diseases, particularly those caused by Aeromonas hydrophila bacteria, are a common issue in these fish. Using immunostimulants to control bacterial diseases can enhance the function of the immune system. Garlic is a natural ingredient that can serve as an effective immunostimulant. This study aims to assess the impact of adding garlic extract to feed on the survival and blood profile of Colossoma macropomum. The research employed an experimental method using a completely randomized design (CRD) with 6 treatments and 3 repetitions. The doses of garlic extract used were P1 (0 g/kg control +), P2 (0 g/kg control -), P3 (10 g/kg), P4 (15 g/kg), P5 (20 g/kg), and P6 (20 g/kg). A total of 180 pomfret fish were used in the study, and they were fed with additional garlic extract for 28 days. Subsequently, the fish were infected with Aeromonas hydrophila bacteria at a specific density. The results of the ANOVA test indicated that the provision of garlic extract had a significant effect on leukocytes (1,99±10388,73 x105 sel/mm3) and erythrocytes (10,10±0,25x106 sel/mm3 ) at H-15 (after bacterial infection) and leukocytes (1,43±9196,33x106 sel/mm3 )at H-28. Furthermore, the use of garlic extract in feed was shown to have a significant effect (P<0.05) on the survival of the fish, with the best survival rate at 83.33±5.77% in treatment P3 (10 g/kg). The study also identified the optimal dose of garlic extract for use as an immunostimulant, which was determined to be P3 (10g/kg).
© The Authors, published by EDP Sciences, 2025
This is an Open Access article distributed under the terms of the Creative Commons Attribution License 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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