| Issue |
BIO Web Conf.
Volume 160, 2025
IV International Conference on Improving Energy Efficiency, Environmental Safety and Sustainable Development in Agriculture (EESTE2024)
|
|
|---|---|---|
| Article Number | 01030 | |
| Number of page(s) | 7 | |
| Section | Sustainable Development in Agriculture | |
| DOI | https://doi.org/10.1051/bioconf/202516001030 | |
| Published online | 12 February 2025 | |
Methods for determining Cytochrome P450 reductase in biological fluids of animals
Federal State Budgetary Educational Institution of Higher Education “Saint Petersburg State University of Veterinary Medicine”, 5, st. Chernigovskaya, Saint Petersburg, 196084, Russia
* Corresponding author: psevdopyos@mail.ru
The study provides a literature review of modern methods for determining Cytochrome P450 reductase. The importance of the enzyme in the body is established. It is shown that Cytochrome P450 reductase determination is used in pharmacology and toxicology. Observed that modern approaches to determining Cytochrome P450 reductase include mass spectrometry, chromatography, electrochemical methods. Spectrometry – allows detection of low concentrations of the substance and study of reaction kinetics. Chromatography (gas, liquid, thin-layer chromatography) is used to separate complex mixtures and determine enzyme activity. Electrochemical methods (voltammetry) help to study the redox transitions of the enzyme. It is confirmed that ELISA is a new era for the Cytochrome P450 reductase determination. Gaps in modern knowledge about Cytochrome P450 reductase are also identified.
© The Authors, published by EDP Sciences, 2025
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