Prevention of subclinical mastitis in lactating cows with a new probiotic preparation

Kazan State Academy of Veterinary Medicine named after N.E. Bauman, Kazan 420029, Republic of Tatarstan, Russia

^* Corresponding author: danai58@yandex.ru

Abstract

The aim of the work is to develop a new domestic probiotic preparation containing strains of microorganisms known as Bacillus subtilis and Bacillus amiloliquefaciens and intended for treatment of cows’ udders, and technology of its application in cattle breeding for prevention of cows with subclinical mastitis and obtaining ecologically clean milk. The composition of microorganism strains was selected on the basis of preliminary microbiological study by sowing on nutrient media. On the basis of scientific and production experience in the conditions of LLC “Soyuz-Agro” of Aznakaevsky district of the Republic of Tatarstan, it was found that for this population of cows the most acceptable was the use of probiotic preparation. It contains compositions of strains of microorganism producers such as Bacillus subtilis strain B-11 in the concentration of 1x1010 CFU/ml and Bacillus amiloliquefaciens of the B-14294 strain in the concentration of 1x1010 CFU/ml. It showed the best activity in relation to the microflora of the studied population of lactating cows. On the background of probiotic preparation application for three months of the experiment in milk of experimental cows the number of somatic cells decreased by 39.6 % and microbial contamination reduced by 38.3 % in comparison with those of control, and economic efficiency per 1 ruble of additional costs amounted to 4.3 rubles.