Polymorphism of g.1637T>G in the IZUMO1 gene as a potential genetic marker for reproductive traits of Donggala cattle (Bos indicus)

¹ Department of Animal Science, Faculty of Agriculture, Djuanda University, Bogor, West Java, Indonesia
² Research Center for Applied Zoology, National Research and Innovation Agency, Bogor, West Java, Indonesia
³ Faculty of Animal Science and Fishery, Tadulako University, Palu, Central Sulawesi, Indonesia
⁴ Department of Agricultural Economics and Animal Production, University of Limpopo, Polokwane, South Africa

^* Corresponding author: widy008@brin.go.id

Abstract

Donggala is one of Indonesia’s local cattle breeds from Central Sulawesi that is kept for meat production. This study was carried out to report the mutation site in the IZUMO1 gene of Donggala cows using forward sequencing methods. Thirty-tree (33) Donggala cows were used in this study as experimental animals. Results showed that 452 bp of the bovine IZUMO1 gene was amplified successfully using a designed primer pair. A mutation site of g.1637T>G (intron 2) was detected in the bovine IZUMO1 gene with a polymorphic informative content (PIC) value of 0.19 (moderate). In addition, the T allele was observed as the dominant allele in the g.1637T>G mutation. Despite this, the frequency of the TT genotype was higher than that of the TG genotype (0,76 vs 0,24). Meanwhile, the GG genotype was absent in the Donggala cows. However, genetic diversity in the g.1637T>G locus was under genetic equilibrium (χ²<3.841). In conclusion, a mutation of g.1637T>G has potency as a genetic marker of reproductive traits in Donggala cattle. In the future, an in-depth study involving a large sample and data records will be essential to clarify this finding.