| Issue |
BIO Web Conf.
Volume 162, 2025
Wildlife-Endangered Species Conservation and Animal Reproduction (WECARe) International Conference 2024
|
|
|---|---|---|
| Article Number | 00014 | |
| Number of page(s) | 7 | |
| DOI | https://doi.org/10.1051/bioconf/202516200014 | |
| Published online | 28 February 2025 | |
Genetic insights: PCR-based sex verification of Limousin bull (Bos taurus) semen
1 Master of Biotechnology Programs, Graduate Schools, Gadjah Mada University, Yogyakarta
2 Research Centre of Biotechnology, Gadjah Mada University, Yogyakarta
3 Departement of Reproduction and Obstetrics, Faculty of Veterinary Medicine, Gadjah Mada University, Yogyakarta
4 Doctor of Veterinary Science Programs, Faculty of Veterinary Medicine, Gadjah Mada University, Yogyakarta
* Corresponding author: uma_vet@ugm.ac.id
The determination of spermatozoa sex is a strategic step in the development of artificial insemination technology. The method of separating spermatozoa carrying female (X) or male (Y) sex chromosomes using Bovine Serum Albumin (BSA) has achieved gender accuracy rates of 70% to 80% based on pregnancy rates. However, this verification method requires a lengthy process to determine the success percentage of sexing. Polymerase Chain Reaction (PCR) testing can be utilized for verification in a relatively short time, requiring a pair of primers to amplify the target DNA region. This study aims to verify sexed spermatozoa using a new primer design for the Limousin cattle X chromosome through PCR testing. The results indicate that sperm separation using the BSA column yielded the PLP1 gene (207 bp). This demonstrates that sperm sexing with the BSA column method can effectively separate sperm carrying X and Y chromosomes, which PCR molecularly verifies.
© The Authors, published by EDP Sciences, 2025
This is an Open Access article distributed under the terms of the Creative Commons Attribution License 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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