Immunoreactivity of VLCRS-infected cows

¹ Kazan State Agrarian University, K. Marx Street 65, Kazan city, 420015, Russia
² Federal Center for Toxicological, Radiation and Biological Safety, Nauchnyi Gorodok-2, Kazan, 420075, Russia
³ Mari State University, 1, Lenin Square, Yoshkar-Ola, 424000, Russia

Abstract

The article describes the results of the ELISA and PCR-RDF blood serum analysis of the env BLV gene in order to study the features of immunoreactivity of infected cattle leukemia virus. The ELISA results showed that 63.8% of the studied blood serum samples from HCV infected cows reacted positively to mycobacterial antigens. The results of a comparative study of PCR-RDF data from DNA analysis of isolates and ELISA indicated that antibodies against antigens of the 4 genotype of VLCRS are more likely to cross-react with antigens of M.Avium, and antibodies against 7 genotype – with antigens of M.Bovis. The presented research results allow us to conclude both about the possible determinant commonality of VLCRS and mycobacteria antigens, and about changes in the immunoreactivity of cows infected with VLCRS. The reason for this heterogeneity and cross-reactivity is, of course, the constant change in titers and spectra of antibodies to HCV in infected animals with the development of the infectious process. And also, the fact that infection of an organism with HCV leads to disruption of the host immune system regulation processes at both the cellular and humoral levels.