| Issue |
BIO Web Conf.
Volume 181, 2025
V International Scientific and Practical Conference “Ensuring Sustainable Development in the Context of Agriculture, Energy, Ecology and Earth Science” (ESDCA 2025)
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|---|---|---|
| Article Number | 01004 | |
| Number of page(s) | 9 | |
| Section | Agriculture | |
| DOI | https://doi.org/10.1051/bioconf/202518101004 | |
| Published online | 19 June 2025 | |
Cloning and bioinformatic analysis of drought-related genes in soybean (Glycine Max L.)
1 National University of Uzbekistan named after Mirzo Ulugbek, Tashkent, Uzbekistan
2 Jizzakh State Pedagogical University, Jizzakh, Uzbekistan
3 Samarkand State University, Samarkand, Uzbekistan
4 Tashkent State Agrarian University, Tashkent, Uzbekistan
* Corresponding author: dilmurodxatamov99@gmail.com
This study investigates the GmSOG1 gene in soybean (Glycine max L.) and its role in drought tolerance. Through bioinformatic analysis, GmSOG1 was identified as a key stress-responsive gene. The CRISPR/Cas9 genome-editing technique was used to target and modify this gene, resulting in the development of mutant lines. Functional analysis showed that GmSOG1 positively regulates antioxidant enzyme activity and the expression of drought-responsive transcription factors, thereby improving plant survival under water deficit conditions. The successful transformation of local soybean cultivars using Agrobacterium tumefaciens and CRISPR/Cas9 confirmed the gene’s relevance in enhancing drought resistance. This research provides a solid foundation for developing resilient soybean varieties and contributes to sustainable agriculture amid climate change challenges.
© The Authors, published by EDP Sciences, 2025
This is an Open Access article distributed under the terms of the Creative Commons Attribution License 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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