Issue |
BIO Web Conf.
Volume 183, 2025
International Conference on Life Sciences and Technology (ICoLiST 2024)
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Article Number | 01029 | |
Number of page(s) | 8 | |
DOI | https://doi.org/10.1051/bioconf/202518301029 | |
Published online | 09 July 2025 |
Analysis of Crystallinity and Morphology of Bacterial Cellulose/Chitosan Based Composite Film
1 Department of Mechanical and Industrial Engineering, Faculty of Engineering, State University of Malang, Jl. Semarang 5, Malang, Indonesia
2 Center of Excellence for Cellulose Composite (CECCom), Department of Mechanical and Industrial Engineering, Faculty of Engineering, State University of Malang, Jl. Semarang 5, Malang 65145, Indonesia
3 Center for Science and Engineering (PSR), LPPM, State University of Malang, Jl. Semarang 5, Malang 65145, Indonesia
4 Faculty of Chemical Engineering Technology, Universiti Malaysia Perlis (UniMAP), Arau 02600, Malaysia
5 Department of Aquatic Resources Management, Faculty of Fisheries and Marine Science, Brawijaya University, Jl. Veteran, Malang, Indonesia.
* Corresponding author: heru.suryanto.ft@um.ac.id
Pineapple peel can be effectively utilized in fermentation process to produce bacterial cellulose (BC). This study uses pineapple peel extract to produce BC, combined with chitosan to form composite films. The study aims to analyze the crystallinity and investigate the surface morphology of BC film with chitosan reinforcement. This method includes the cellulose synthesis through the fermentation process, alkalization, and defibrillation using high-pressure homogenization on BC and the synthesis of BC film with chitosan content of 2.5%, 5%, 7.5%, and 10% then casting and drying at 50°C for 20 hours. Crystallinity was analyzed using X-ray diffraction, and surface morphology was observed using a scanning electron microscope. Results showed an influence of adding chitosan on the surface morphology and crystallinity of the BC composite film. The diffraction pattern indicates dominant peaks with the addition of chitosan were achieved at about 11°, 14°, 16°, 22°, and 26°. The addition of chitosan reduces the crystallinity index of BC film from BC Control 83.4 % to 75.4% after adding chitosan of 10%. Surface morphology shows a smooth surface of film on BC film, but rougher after adding chitosan to make the surface corrugated. Future studies will explore the benefits of film in biopackaging applications.
© The Authors, published by EDP Sciences, 2025
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