Bioactive Compounds, Tyrosinase Inhibitor and Antimicrobial Potential of Moringa oleifera L. seeds extract

¹ Department of Biochemistry, Faculty of Medicine, Universitas Kristen Indonesia
^2,3 Department of Microbiology, Faculty of Medicine, Universitas Kristen Indonesia

^* Corresponding author: trini.suryowati@uki.ac.id

Abstract

Moringa oleifera L. seeds contain flavonoids, steroids and alkaloids, known to act as antioxidants. The purpose of this study was aimed at investigating the biologically active compounds, tyrosinase inhibition and antimicrobial activity of Moringa seeds against Gram-positive bacteria Streptococcus pneumoniae ATCC 49136 and Gram-negative bacteria Escherichia coli ATCC 25922. Identification of the chemical compounds was conducted using GC-MS technique, the tyrosinase inhibitory affects test was measured with spectrophotometry and the antimicrobial activity test was performed in vitro using the agar disc diffusion assay. The bioactive compound evaluation of Moringa seed extract confirmed the presence of 9 -Octadecenoic acid (50.67) ; Oleyl oleate (8.05%); 1-(1-Butoxy-2-propoxy) -2-propanol TMS derivative (4.12%) ; 4-Trifluoromethylbenzoic acid, tetradecyl ester (3.57%); 9-Octadecenoic acid (Z)-, oxiranyl methyl ester (3.26%). IC50 values for inhibition of tyrosinase seed extract were 365.32 ppm and the kojic acid standard was 26.51 ppm. The antimicrobial activity was performed against bacteria as it showed inhibition zone. These results indicated that Moringa oleifera L. seed extract exerted potent antibacterial activity and is a promising candidate in the treatment of skin hyperpigmentation disorders.