Bidara (Ziziphus mauritiana Lam.) leaves as Cytotoxic agent Against Hela Cervical Cancer Cell Line

¹ Department of Biochemistry and Physiology, Faculty of Medicine and Health Sciences, Warmadewa University, Indonesia
² Department of Pathology and Anatomy, Faculty of Medicine and Health Sciences, Warmadewa University, Indonesia
³ Department of Histology and Anatomy, Faculty of Medicine and Health Sciences, Warmadewa University, Indonesia
⁴ Undergraduate Student, Medicine Major, Faculty of Medicine and Health Sciences, Warmadewa University, Indonesia
⁵ Department of Microbiology and Parasitology, Faculty of Medicine and Health Sciences, Warmadewa University, Indonesia

^* Corresponding author: donald.calista@gmail.com

Abstract

Cancer remains as one of the leading cause of fatal disease globally. Though preventable by vaccines, cervical cancer remains the second most common type of cancer diagnosed in women. Current cancer treatments are costly, time-consuming, and gave various side effects. Bidara plants contain secondary metabolites and antioxidants that are beneficial for health. This study aims to examine the anticancer effects of bidara leaves methanolic extract on HeLa cervical cancer cells. We incubate HeLa cells with different doses of bidara leaf extract (0 μg/ml, 15.6 μg/ml, 31.3 μg/ml, 62.5 μg/ml, 125 μg/ml, 250 μg/ml, 500 μg/ml, and 1000 μg/ml) for 24 hours and determined the percent viability of cells using MTT assay with absorbancy at 595nm. We found that bidara leaves methanolic extract has IC₅₀ of 23 μg/ml which means that bidara leaves has strong anticancer potential against Hela cancer cell line. We did in-silico analysis on two leading compound in bidara leaves extract, desmethylclomipramine and demeclocycline where we find that those two compounds have binding affinity with BCL-2, an important protein involved in apoptosis process with molecular affinity energy ranging from -6.5 kcal/mol and -7.1 kcal/mol respectively which indicates good affinity binding between both compounds and BCL2.