Whole Genome Sequencing of A Healthy Sundanese Individual As an Initial Study for Sundanese Population Genetic Reference Foundation

¹ Biochemistry, IPB University, 16680 Bogor, Indonesia
² Satriabudi Dharma Setia Foundation, Tangerang, Indonesia

^* Corresponding author: dimasandrianto@apps.ipb.ac.id

Abstract

Sundanese is the second-largest tribe in Indonesia. Despite its substantial and diverse population, the application and database of whole-genome sequencing in Indonesia, especially among Sundanese individuals, are limited. This study aims to establish a genetic reference for Indonesia by presenting phenotypic and clinical data from Whole-Genome Sequencing of Sundanese individuals. Method selection of the subject based on physical health and three-generation Sundanese ancestry. Results show that clinical assessments revealed a body weight of 55.3 kg, a height of 171.8 cm, a BMI of 18.7, a fasting blood glucose level of 69 mg/dL, a cholesterol level of 141 mg/dL, and a uric acid level of 3.3 mg/dL. Anthropometric measurements, including nasal index, classified the nose as broad. DNA extraction using proteinase K yielded 28 ng/μL, with mechanical shearing recovering 40.04% of the extracted DNA. A total of 2,215,390 reads with an average coverage of 33.9x were obtained from the alignment process and genotype data, which can be used for downstream whole-genome sequencing analysis. These genotype data support downstream WGS analysis, contributing to a foundational genomic resource for Indonesia’s Sundanese population and can be used as a basis for studying unique genetic variants and clinical implementation in the future.