The effect of seeds soaking duration in alcohol of In vitro germination Sesbania grandiflora

Department of Animal Science, Universitas Andalas, Indonesia

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Abstract

Turi (Sesbania grandiflora) is a nutrient-rich legume. All parts of the turi plant can be used as a source of animal feed. Turi plants have hard seeds, which is a limitation in cultivation and are relatively slow to regrow after being cut. This study aims to determine the sterilization concentration of turi seeds using alcohol to accelerate the in vitro germination. This research uses an experimental method consisting of 6 treatments, namely P0: no alcohol soaking, P1: soaked in 70% alcohol for 5 minutes, P2: 10 minutes, P3: 15 minutes, P4: 20 minutes, and P5: 25 minutes. The variables measured are initial germination time (days), germination rate (%), and contamination percentage (%). The results of the study showed that the average germination time ranged from 0 to 13.00 days, the average germination rate ranged from 0 to 46.00%, and the contamination percentage ranged from 0% to 45%. The diversity analysis results on the treatment of soaking turi seeds in alcohol regarding germination time, germination rate, and contamination percentage could not be statistically tested because the data did not meet the requirements for representative data for statistical analysis, as less than 50% of the seeds germinated.