Determination of Total Phenol Compound Content in Mango Mistletoe Leaves (Dendrophthoe pentandra (L.) Miq) Using the UV-Vis Spectrophotometry Method

Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Islam Malang, Indonesia

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Abstract

Mango mistletoe leaves (Dendrophthoe pentandra (L.) Miq) are parasitic plants traditionally used as medicine due to their secondary metabolites and strong antioxidant activity. This study aimed to determine the total phenolic content and assess the effect of concentration on absorbance values using UV-Vis spectrophotometry, as a specific parameter for the development of herbal products. The research included sample preparation, extraction, preparation of gallic acid standards (10, 15, 20, and 25 ppm), and measurement of absorbance at 764 nm. Ethanol extracts of mango mistletoe leaves were evaluated at concentrations of 100, 200, 300, and 1000 ppm. The gallic acid standard curve showed a regression coefficient of R² = 0.994, confirming its reliability. The total phenolic content obtained was 77.19 ± 0.44 mg/mL (100 ppm), 125.05 ± 0.45 mg/mL (200 ppm), 159.58 ± 2.00 mg/mL (300 ppm), and 228.73 ± 33.06 mg/mL (1000 ppm). The results demonstrated that phenolic content increased proportionally with concentration. In conclusion, mango mistletoe leaves contain significant levels of phenolic compounds, supporting their potential as a raw material for herbal products with vigorous antioxidant activity and fulfilling the criteria as a parameter for herbal product development.