| Issue |
BIO Web Conf.
Volume 209, 2026
The 1st International Conference on Biological Technology for Sustainable Nature (IC-BioTEStA 2025)
|
|
|---|---|---|
| Article Number | 01006 | |
| Number of page(s) | 9 | |
| Section | Fundamental Biological Sciences | |
| DOI | https://doi.org/10.1051/bioconf/202620901006 | |
| Published online | 09 January 2026 | |
Analysis of Bioactive Compounds and DPPH Radical Inhibition Activity of Hydroethanolic Extracts from Muouna pruriens Seeds
1,3 Study Program of Biology, Universitas Islam Malang, Malang, 65144, Indonesia
2 Chemistry Department, Universitas Jember, Jember, 68121, Indonesia
1 Corresponding author: This email address is being protected from spambots. You need JavaScript enabled to view it.
Mucuna pruriens is a medicinal plant known to contain various bioactive compounds, including L-DOPA, alkaloids, phenolics, and tannins, with potential as a natural antioxidant. This study aimed to evaluate the characteristics of Mucuna pruriens seed simplicia and its hydroethanolic extract, identify its secondary metabolites, and determine its antioxidant activity. Simplicia were characterized through moisture and ash content analysis, and extraction was carried out using maceration with hydroethanol (1:1) solvent. The results showed a moisture content of 2% and an ash content of 2.1%, meeting the standards of the Indonesian Herbal Pharmacopoeia. The extract yield was 17.6%, indicating good extraction efficiency. Phytochemical screening revealed the presence of alkaloids, phenolics, tannins, and triterpenoids. Antioxidant activity was assessed using the DPPH method, yielding an IC50 value of 2.18 ppm, indicating a powerful antioxidant category. These findings suggest that the hydroethanolic extract of Mucuna pruriens seeds has high potential as a natural antioxidant source for health and cosmetic applications.
Key words: Antioxidant activity / bioactive compounds / DPPH assay / hydroethanolic extract / Mucuna pruriens
© The Authors, published by EDP Sciences, 2026
This is an Open Access article distributed under the terms of the Creative Commons Attribution License 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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