Antidiabetic Potency of Pulutan (Urena lobata) Leaf Fraction and its Molecular Docking Analysis

Center for the Study of Islamic Medical Integration, Bioinformatics, and Indonesian Traditional Medicine, Universitas Islam Malang, Malang 65144, Indonesia

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Abstract

Urena lobata is medicinal plant used to cure Diabetes mellitus traditionally. However, the phytoconstituent regulating blood glucose levels and their mechanism remain unexlpored. The study aim to evaluate antidiabetic potency of U.lobata leaf fractions. Ethanol solvent is used to extract U. lobata leaf by kinetic maceration methods therefore the concentrated extract was fractionated using n-hexane (Fraction A), n-butanol (Fraction B) and aqueous solvent (Fraction C) respectively. The anti-diabetic potency U.lobata was examined by oral glucose tolerance test. Blood glucose level of rat were measured after loading of glucose at the certain time and Area Under Curve (AUC) integrated were calculated using trape zoidal methods. Data was analyzed using one way ANOVA and continued with LSD test (p<0.05). Molecular docking was conducted by the PyRx 0.8 program after phytoconstituent identification of U.lobata extract using LC-HRMS. Ethanolic extract, fraction A, B and C decreased glucose AUC about 16 %, 8 %, 11% and 23% respectively compared to control group (p<0.05). Meanwhile, glibenclamid as reference drugs decreased glucosa AUC about 30 % and stronger than extract of U. lobata and its fractions. (+)-ar-Turmerone is predicted as potential compound in U.lobata leaf extract as anti-diabetic by binding on SUR1 based on in silico approach.