Antibacterial Activity of Endophyte Bacteria Isolation from Mangrove Leaves Scyphiphora hydrophyllacea

Biology Sciences, Faculty of Mathematics and Natural Sciences, 98314 Papua University, Manokwari, West Papua, Indonesia

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Abstract

Antibacterial activity testing is a fundamental method for evaluating the ability of bacterial isolates to inhibit the growth of pathogenic microorganisms. This study aimed to identify and describ endophytic organisms by isolating bacteria from the mangrove leaves of Scyphiphora hydrophyllacea Gaertn, and to assess their antibacterial potential against Staphylococcus aureus and Escherichia coli. Antibacterial assays were conducted using metabolites extracted from the endophytic isolates, employing both Kirby-Bauer test and Minimum Inhibitory Concentration (MIC) methodologies. Of the 12 bacterial isolates obtained, seven exhibited inhibitory activity against S. aureus and E. coli. Notably, isolates SH1 and SH2 demonstrated the most pronounced inhibitory zones, varying from 10 mm to 12 mm. MIC analysis revealed that both SH1 and SH2 effectively suppressed E. coli growth at a concentration of 1.56 ug/ml, as indicated by the reduced absorbance levels. For S. aureus, the MIC values were determined to be 12.5 ug/ml SH1 and 3.125 ug/ml SH2. These findings suggest that endophytic bacteria isolated from S. hydrophyllacea possess promising antibacterial properties and warrant further investigation for their potential therapeutic applications. Based on the identification results, bacterial isolates SH1, SH2, and SH7 were identified as Amphibacillus sp., Sporolactobacillus sp., and Bacillus sp.