Comparative Phytochemical Profiling and Evaluation of the Antibacterial and Antifungal Efficacy of Stevia rebaudiana Bertoni and Marrubium Vulgare L. Hydroethanolic Extracts

¹ URL CNRSTN15, Human Pathology, Biomediciiie and Environment Laboratory, Faculty of Medicine, Pharmacy and dentistry, Sidi Mohamed Ben Abdellah University, Fez, Morocco
² Laboratory of Functional Ecology and Engineering Environment, Faculty of Sciences and Techniques, Sidi Mohamed Ben Abdellah University, Fez, Morocco
³ Laboratory of Applied Organic Chemistry, Faculty of Science and Techniques, Sidi Mohamed Ben Abdellah University, Fez, Morocco
⁴ Higher Institute of Nursing professions and Health Techniques of Fez-Meknes, Morocco

^* Corresponding author: This email address is being protected from spambots. You need JavaScript enabled to view it.

Abstract

This study evaluates and compares the phytochemical profiles and antimicrobial efficacy of Marrubium vulgare L. and Stevia rebaudiana Bertoni hydroethanolic extracts. Using HPLC-ESI-MS/MS, we identified including marrubiin, verbascoside, and steviol glycosides. We tested these extracts against four bacterial strains and Candida albicans. Gram-positive strains exhibited significant susceptibility to M. vulgare (MIC 0.78-1.56 mg/mL). In contrast, S. rebaudiana showed superior antifungal activity against C. albicans (MIC 1.56 mg/mL). These distinct profiles highlight the specific potential of M. vulgare as an antibacterial and S. rebaudiana as an antifungal agent in natural medicine.