Biofabrication of Zinc Oxide Nanoparticles Using Streptomyces cyaneofuscatus

¹ Biotechnology Program, Basic and Applied Science Institute, Egypt Japan University of Science and Technology (E-JUST), New Borg El-Arab City, Alexandria 21934, Egypt.
² Botany and Microbiology Department, Faculty of Science, Tanta University, Tanta 31527, Egypt.
³ Biochemistry Department, Faculty of Science, Ain Shams University, Cairo 11566, Egypt.
⁴ Environmental Biotechnology Department, Genetic Engineering and Biotechnology Research Institute (GEBRI), City of Scientific Research and Technological Applications (SRTA-City), New Borg El-Arab City, Alexandria 21934, Egypt.
⁵ Biochemistry Department, Faculty of Agriculture, Cairo University, Giza 12613, Egypt.

^* Corresponding author: Manar K. Abd Elnabi Email: This email address is being protected from spambots. You need JavaScript enabled to view it.

Abstract

This article discusses a simple, cost-effective, and biocompatible method for biosynthesis of ZnONPs. For the first time, Streptomyces cyaneofuscatus cell free filterate, as a reducing and stabilizing agent, was used to prepare ZnONPs using zinc acetate as metal precursor. The prepared ZnONPs were characterized using multiple analytical techniques. The UV-Vis spectrum analysis of the biosynthesized ZnONPs exhibited an absorption peak at 375 nm. Fourier-transform infrared (FT-IR) spectroscopy suggested the presence of different functional groups of microbial biomolecules. XRD verified hexagonal wurtzite crystalline structure of ZnONPs. The zeta potential analysis showed good stability of ZnONPs (-26.6 mV). Furthermore, the EDX analysis explained the 73.11 % of zinc (Zn) and 26.89% of oxygen (O) elemental structure of fabricated ZnONPs which indicated the successful fabrication of ZnONPs by S. cyaneofuscatus.